This portal has not been updated since October 2011. Please use the new PC2 web services.
Send us your feedback.  Sign up for Pathway Commons announcements.  RSS Logo RSS Feed

Searched for: REACTOME AND Processive synthesis on the lagging strand [All Organisms, All Data Sources]

Your search has found 15 relevant records:

Narrow Results:

Pathways (15)Molecules (26)
Showing Results 1 - 10 of 15  | Next 10
0%0%
Pathway: Processive synthesis on the lagging strand  from Reactome  [55 molecules]
The key event that allows the processive synthesis on the lagging strand, is polymerase switching from pol alpha to pol delta, as on the leading strand.

Summary:  The key event that allows the processive synthesis on the lagging strand, is polymerase switching from pol alpha to pol delta, as on the leading strand. However, the processive synthesis on the lagging strand proceeds very differently. DNA synthesis is discontinuous, and involves the formation of short fragments called the Okazaki fragments. During the synthesis of Okazaki fragments, the RNA primer is folded into a single-stranded flap, which is removed by endonucleases. This is followed by the ligation of adjacent Okazaki fragments.

  • ... Processive synthesis on the lagging strand ...
  • ... Reactome ...
  • ... reactome.
  • NCBI_TAXONOMY ...
  • The key event that allows the processive synthesis on the lagging strand, is polymerase switching from pol alpha to pol delta, as on the leading strand. ... However, the processive synthesis on the lagging strand proceeds very differently. ... DNA synthesis is discontinuous, and involves the formation ...
0%0%
Pathway: Telomere C-strand (Lagging Strand) Synthesis  from Reactome  [63 molecules]
Reviewed: Price, C, 2006-07-13 18:33:38.

Summary:  Reviewed: Price, C, 2006-07-13 18:33:38

  • Telomere C-strand (Lagging Strand) Synthesis ...
  • ... Reactome ...
  • ... reactome.
  • NCBI_TAXONOMY ...
  • Telomere C-strand (Lagging Strand) Synthesis. ... strand is synthesized discontinuously. ... This strand is called the lagging strand. ... Although the polymerase switching on the lagging strand is very similar to that on the leading strand, the processive. ..., called Okazaki fragments are synthesized on the RNA-DNA primers first. ... Strand-displacement synthesis ...
0%0%
Pathway: Lagging Strand Synthesis  from Reactome  [65 molecules]
Due to the antiparallel nature of DNA, DNA polymerization is unidirectional, and one strand is synthesized discontinuously.

Summary:  Due to the antiparallel nature of DNA, DNA polymerization is unidirectional, and one strand is synthesized discontinuously. This strand is called the lagging strand. Although the polymerase switching on the lagging strand is very similar to that on the leading strand, the processive synthesis on the two strands proceeds quite differently. Short DNA fragments, about 100 bases long, called Okazaki fragments are synthesized on the RNA-DNA primers first. Strand-displacement synthesis occurs, whereby the primer-containing 5'-terminus of the adjacent Okazaki fragment is folded into a single-stranded flap structure. This flap structure is removed by endonucleases, and the adjacent Okazaki fragments are joined by DNA ligase.

  • ... Lagging Strand Synthesis ...
  • ... Reactome ...
  • ... reactome.
  • NCBI_TAXONOMY ...
  • Due to the antiparallel nature. ... is called the lagging strand. ... Although the polymerase switching on the lagging strand is very similar to that on the leading strand, the processive synthesis on the two strands proceeds quite. ... Strand-displacement synthesis occurs, whereby the primer-containing 5'-terminus ...
0%0%
Pathway: Processive synthesis on the C-strand of the telomere  from Reactome  [39 molecules]
Reviewed: Price, C, 2006-07-13 18:33:38.

Summary:  Reviewed: Price, C, 2006-07-13 18:33:38

  • ... Processive synthesis on the C-strand of the telomere ...
  • ... Reactome ...
  • ... reactome.
  • NCBI_TAXONOMY ...
  • ... Processive synthesis on the C-strand. ... and lagging strand synthesis.
  • ... Reactome stable identifier. ... Use this URL to connect to the web page of this instance in Reactome: http://www. ...reactome. ... the processive synthesis of a short run of DNA called an Okazaki fragment begins. ... DNA synthesis ...
0%0%
Pathway: Polymerase switching on the C-strand of the telomere  from Reactome  [29 molecules]
Reviewed: Price, C, 2006-07-13 18:33:38.

Summary:  Reviewed: Price, C, 2006-07-13 18:33:38

  • ... initiation of leading and lagging strand synthesis.
  • Polymerase switching on the C-strand of the telomere ...
  • ... Reactome ...
  • ... reactome.
  • NCBI_TAXONOMY ...
  • Polymerase switching on the C-strand. ... polymerase delta binds and catalyzes the processive synthesis of DNA.
  • After the primers are synthesized on the G-Rich strand, Replication ...
0%0%
Pathway: Polymerase switching  from Reactome  [29 molecules]
After the primers are synthesized, Replication Factor C binds to the 3'-end of the initiator DNA to trigger polymerase switching.

Summary:  After the primers are synthesized, Replication Factor C binds to the 3'-end of the initiator DNA to trigger polymerase switching. The non-processive nature of pol alpha catalytic activity and the tight binding of Replication Factor C to the primer-template junction presumably lead to the turnover of the pol alpha:primase complex. After the Pol alpha-primase primase complex is displaced from the primer, the proliferating cell nuclear antigen (PCNA) binds to form a "sliding clamp" structure. Replication Factor C then dissociates, and DNA polymerase delta binds and catalyzes the processive synthesis of DNA.

  • ... and delta during initiation of leading and lagging strand synthesis.
  • Kwong, AD. ... polymerase delta binds and catalyzes the processive synthesis of DNA.
  • ... Use this URL to connect to the web page of this instance in Reactome: http://www. ...reactome.
  • ... Reactome ...
  • ... reactome.
  • ... -processive nature of pol alpha catalytic activity and the tight binding of Replication Factor C ...
0%0%
Pathway: Leading Strand Synthesis  from Reactome  [29 molecules]
The processive complex is responsible for synthesizing at least 5-10 kb of DNA in a continuous manner during leading strand synthesis.

Summary:  The processive complex is responsible for synthesizing at least 5-10 kb of DNA in a continuous manner during leading strand synthesis. The incorporation of nucleotides by pol delta is quite accurate. However, incorporation of an incorrect nucleotide does occur occasionally. Misincorporated nucleotides are removed by the 3' to 5' exonucleolytic proofreading capability of pol delta.

  • Leading Strand Synthesis ...
  • ... Reactome ...
  • ... reactome.
  • NCBI_TAXONOMY ...
  • The processive complex is responsible for synthesizing at least 5-10 kb of DNA in a continuous manner during leading strand synthesis. ... this URL to connect to the web page of this instance in Reactome: http://www. ...reactome.
  • ... REACTOME DATABASE ID ...
0%0%
Pathway: DNA Damage Bypass  from Reactome  [15 molecules]
In addition to various processes for removing damaging lesions from the DNA, cells have developed specific mechanisms for tolerating unexcised damages during the replication of the genome.

Summary:  In addition to various processes for removing damaging lesions from the DNA, cells have developed specific mechanisms for tolerating unexcised damages during the replication of the genome. Such processes are collectively called DNA damage bypass pathways. Several proteins including novel Y-family polymerases that have been recently identified in multitude of organisms are involved in this process.

  • ... Reactome ...
  • ... reactome.
  • BR Translesion synthesis (TLS) or replicative bypass of damaged bases that are known to arrest high fidelity, highly processive polymerases involved. ... synthesis by these 3 enzymes is annotated here. ... Complete details of damage recognition and discrimination. ... to the web page of this instance in Reactome: http://www. ...reactome.
0%0%
Pathway: RNA Polymerase II Transcription Elongation  from Reactome  [73 molecules]
The mechanisms governing the process of elongation during eukaryotic mRNA synthesis are being unraveled by recent studies.

Summary:  The mechanisms governing the process of elongation during eukaryotic mRNA synthesis are being unraveled by recent studies. These studies have led to the expected discovery of a diverse collection of transcription factors that directly regulate the activities of RNA Polymerase II and unexpected discovery of roles for many elongation factors in other basic processes like DNA repair, recombination, etc. The transcription machinery and structural features of the major RNA polymerases are conserved across species. The genes active during elongation fall under different classes like, housekeeping, cell-cycle regulated, development and differentiation specific genes etc. The list of genes involved in elongation has been growing in recent times, and include: -TFIIS,DSIF, NELF, P-Tefb etc. that are involved in drug induced or sequence-dependent arrest - TFIIF, ELL, elongin, elongator etc. that are involved in increasing the catalytic rate of elongation by altering the Km and/or the Vmax of Pol II -FACT, Paf1 and other factors that are involved chromatin modification - DNA repair proteins, RNA processing and export factors, the 19S proteasome and a host of other factors like Spt5-Spt5, Paf1, and NELF complexes, FCP1P etc. (Arndt and Kane, 2003). Elongation also represents processive phase of transcription in which the activities of several mRNA processing factors are coupled to transcription through their binding to RNA polymerase (Pol II). One of the key events that enables this interaction is the differential phosphorylation of Pol II CTD. Phosphorylation pattern of CTD changes during transcription, most significantly at the beginning and during elongation process. TFIIH-dependent Ser5 phosphorylation is observed primarily at promoter regions while P-Tefb mediated Ser2 phosphorylation is seen mainly in the coding regions, during elongation. Experimental evidence suggests a dynamic association of RNA processing factors with differently modified forms of the polymerase during the transcription cycle. (Komarnitsky et al., 2000). [Komarnitsky et al 2000, Arndt & Kane 2003, Shilatifard et al 2003]

  • ... Reactome ...
  • ... reactome. ... The mechanisms governing the process of elongation during eukaryotic mRNA synthesis are being. ... Elongation also represents processive phase. ..., JW, Conaway, RC, 2003--0-9- ...
  • ... Reactome stable identifier. ... Use this URL to connect to the web page of this instance in Reactome: http://www. ...reactome.
0%0%
Pathway: HIV-1 Transcription Elongation  from Reactome  [87 molecules]
Edited: Matthews, L, 2005-07-26 23:29:22.

Summary:  Edited: Matthews, L, 2005-07-26 23:29:22

  • ... Reactome ...
  • ... reactome.
  • ... is incomplete with non-processive polymerases disengaging from the proviral DNA template prematurely.
  • GENE_ONTOLOGY ...
  • ... Reactome stable identifier. ... Use this URL to connect to the web page of this instance in Reactome: http://www. ...reactome.
  • ... REACTOME ...